Senior Research Associate. Department of Chemistry, Institute for Lasers Photoni Resume
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Resume:
Dr. Adela C. Bonoiu
EDUCATION
MSc l Biochemistry and Molecular Biology, University of Bucharest, Dept of Biochemistry, Romania()
PhD Biochemistry University of Biology, University of Bucharest, Dept of Biochemistry, Romania ()
CAREER GOALS
Excellent Nanotechnology Biomedical and Molecular Medicine Research Scientist seeks for a new permanent career path. Hands-on experience in biomedical science, molecular medicine, space life sciences and biotechnology. Aim to work in compatible Biotechnology and/or Pharmaceutical Industry R & D labs as well as in Science Labs.
LIFE SKILLS
Excellent problem solving and negotiation skills. Works well in multicultural environments;
Optimistic and positive view to life and future and mentoring attitude towards colleagues;
Encouraging team player – want to seek opportunities to team’s best and advancements;
My Leadership model is based on my own example – it is doing more, talking less – or walk your talk;
Multitasker and achiever, can make things to happen, likes to work in multiple projects;
WORK EXPERIENCE
/ - Present Senior Research Associate. Department of Chemistry, Institute for Lasers Photonics and Biophotonics, SUNY Buffalo, NY
- Preparation scientific presentation and proposals
- Investigate the effect of gene silencing on chronic pain behavior
- Investigate the effect of ssRNA nanoplexes on activation of the innate immune response.
/ - / Postdoctoral Fellow . Department of Chemistry, Institute for Lasers Photonics and Biophotonics, SUNY Buffalo, NY
- Development of nanoformulation for gene silencing in rat hippocampus.
- Identification and selection of nanoparticles as gene carrier for gene silencing or augmentation in vitro and in vivo.
- Investigate the effect of DARPP- silencing in improving the addictive behavior
/-/ Research Scientist, Department of Biochemistry, University at Bucharest
- Investigation the effect of UVA and UVB on the morphology of skin cells;
SPECIAL COMPETENCIES AND INTERESTS
Nanotechnology with medical application: gene transfection using nanoparticles formulation, methods for testing the gene transfection in vivo and in vitro, cell toxicity after nanoparticles treatment.
Cell culture: primary cells line (fibroblast, kerationocyte, melanocyte, neurons), brain blood barriers constructs, BMVEC, COS ,, HEK, HeLa cells.
Molecule biology: DNA recombination, plasmid/retroviral plasmid construction, subcloning, sequencing of PCR, stable/transient transfection by Lipofectamine, FuGene , retrovirus, Nucleofector and Electroporation, siRNA, PCR, RT-PCR, situ hybridization, DNA and RNA isolation.
Cell biology: Cell lines/primary cell culture, cell growth viability curve, cell apoptosis and differentiation assays, immunofluorescent double/triple staining, cell sorting by FACS, gel zimography;
Microscopy: Light microscopy, fluorescent/laser confocal microscopy, Dark Field Microscopy, Raman and CARS microscopy
Virology: Influenza Virus plaque assay
Immunology: Culture and transfection, luciferase assay, ChIP assay, ELISA, Western blotting, immunohistochemistry, flow cytometry
LANGUAGE SKILLS
Romanian, mother language (excellent)
English, spoken and written (excellent)
French, spoken and written (good)
Russian, basic
PUBLICATIONS (Display from a list of publications and one US patent application)
Adela C. Bonoiu, Earl J. Bergey, Hong Ding, Rui Hu, Rajiv Kumar, Ken-Tye Yong, Paras N. Prasad, Supriya Mahajan, Kelly E. Picchione, Arin Bhattacharjee, Tracey A. Ignatowski Gold Nanorod-siRNA Induces Efficient In Vivo Gene Silencing in the Rat Hippocampus. Accepted Future Nanomedicine (On-line June ).
Chakravarthy, K.V., Bonoiu, A.C., Davis, W.G., Rajan, P., Ding, H, Hu., Rui, Bowzard, J.B., Bergey, E.J., Katz, M.J., Knight P.R., Sambhara, S., & Prasad, P.N. (). Gold nanorod delivery of an ssRNA immune activator inhibits pandemic HN influenza viral replication. PNAS (), -;
Bonoiu, A.C., Mahajan, S.D., Ding, H., Roy, I., Yong, K.Y., Kumar, K., Hu, R., Bergey, E.J., Schwartz, S.A., & Prasad, P.N., (). Nanotechnology approach for drug addiction therapy: gene silencing using delivery of gold nanorod-siRNA nanoplex in dopaminergic neurons, ():.
Bonoiu, A.C., Mahajan, S.D., Ye, L., Kumar, R., Ding, H., Yong, K.Y., Roy, I., Aalinkeel, R., Nair, B., Reynolds, J.L., Sykes, D.E., Imperiale, M.A., Bergey, E.J., Schwartz, S.A., & Prasad, P.N., (). MMP- gene silencing by a quantum dot-siRNA nanoplex delivery to maintain the integrity of the blood-brain barrier. Brain Res, , -.
REVIEWER FOR JOURNALS
Nanoscale , Molecular Biosystem ,Analyst , Soft Matter , Journal of Materials Chemistry, Integrative Biology
MEMBERSHIPS
American Chemical Society, American Pain Society
