Graduate Student, Masters in Biotechnology Resume
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Resume:
Vidhi A. Khandelwal
Cherry Street, #
College Station,TX
Phone:
E-mail:
OBJECTIVE
Master’s level graduate in Biotechnology with Business skills seeking a full-time position in Biotechnology/Pharmaceutical research
EDUCATION
Texas A&M University, College Station, TX Master of Science in Biotechnology, Minor in Business, Minor in Business GPA:./. Grad: May
KIT College of Engineering, Shivaji University, Kolhapur, India Bachelor in Engineering, Biotechnology
GPA: ./ .May
Relevant subjects: Molecular and Cell Biology, Genetics, Proteomics, Microbiology, Immunology, Biochemistry, Laboratory work , Technical Writing , Financial Accounting, Finance, Marketing, Management and Project Management.
WORK EXPERIENCE
R&D Intern, Sigma Aldrich, St Louis, MO Exploring protein enrichment strategies compatible with mass spectrometry for novel biomarker discovery from human plasma
June - Aug
•Coupled antibody-based enrichment of target analytes with immunodepletion approaches and detected proteins from human plasma previously unseen by mass spectrometry, thereby enabling the possible discovery of new biomarkers
•Tested two different enrichment strategies and their compatibility with mass spectrometry workflows, by spiking proteins into plasma and subsequently enriching them either at protein or peptide level.
•Analyzed the enriched samples via mass spectrometry and the pitfalls of each approach were elucidated.
•Preliminarily, limit of detections as low as ng/mL was uncovered and areas for future optimization were identified to push this limit further.
Graduate Teaching Assistant, Texas A&M University, TX
Aug - Dec
•Conducted the introductory microbiology laboratory course for undergraduate students.
•Created and maintained detailed study plan, organized review sessions, proctored and graded exams and maintained student records.
ACADEMIC PROJECTS
Generation of shuttle vector using the gateway cloning system to produce histidine-tagged recombinant protein Texas A&M University, College Station, TX
Aug - Dec
•Constructed a shuttle vector, pENTR-His-ccdB, that expedites the expression of histidine-tagged recombinant protein and allows testing of different expression systems for uncharacterized proteins.
•Two histidine clones, produced by encoding histidines and tobacco etch virus protease upstream of the recombinant site, was expressed using the newly engineered vector, purified through affinity chromatography and analyzed by western blotting.
Immuno-proteomic analysis of membrane vesicles (MV) from Salmonella enteric serovar Typhimurium (Stm)
Texas A&M Health Science Center, College Station, TX
Jan - May
•Identified protein components in Salmonella MVs, which are an additional means to induce pathogen-specific immunity, by immunoblotting, two dimensional gel electrophoresis and mass spectrometry
•Compared MV of wild type and mutant strains and determined components in mutants that contribute to immunogenicity against Salmonella.
Infection of African Green Monkey Kidney cells by rotavirus for expression of non-structural protein (NSP).
Texas A&M University, College Station,TX
Jan - May
•Expressed rotavirus endotoxin NSP by infecting African Green Monkey Kidney cells with rotavirus
•Measured infectivity by immunofluorescence technique and analyzed the expressed protein by western blot
TECHNICAL SKILLS
•Molecular Biology: DNA/RNA isolation & characterization, southern blotting, northern blotting, cloning, rDNA technology, PCR and sequencing
•Cell Culture: Isolation and maintenance of mammalian ,bacterial and yeast cells ,media optimization, transfection and culture preservation
•Proteomics: Protein quantitation, SDS-PAGE, ELISA, western blotting, immunoprecipitation,UV/Vis spectrometry, immunoaffinity chromatography
•Microbiology: Media preparation, slide preparation, bacteria culturing, isolation and quantification, transformation, and aseptic techniques
•Computer: MS office(Word, Excel,Power Point,Outlook and Project) , Prism, Softmax Pro , BLAST, EndNote, Macintosh & Windows OS.
LEADERSHIP AND HONORS
•Graduate Teaching Assistantship, Texas A&M University, TX
Aug - Dec
•Vice President of the Biotechnology Society, Texas A&M University, TX
Sept - Sept
•Member of volunteering services -National Service Scheme (NSS),India
July - May
WORK AUTHORIZATION
Eligible to work in U.S.A. through Optional Practical Training
