Technician Assistant Resume
LOG IN OR REGISTER TO CONTACT ME
This button will open the login/register page in a new tab. After logging in, come back to this page and refresh your browser.
Resume:
Sylwia Szczur
Wroclaw University of Technology, Poland
- Master and Batchelor\'s Degree in Biotechnology with \'\'Medicinal Chemistry\'\'
specialisation
Main subjects: Inorganic and Organic Chemistry, Biology, Maths, Phisic,
Cellular and Molecular Biology, Computer science, Microbiology, Biochemistry,
Genetics, Biotechnology, Molecular Biology, Genetic engineering, Medicinal
Biochemistry, Drug design, Combinatorial chemistry, Plant and Animal tissue
cultures, Molecular Modeling, Multi-step organic synthesis,
Polish Academy of Sciences
The project was focused on membrane protein from Streptomyces coelicolor – CpkF, which we believe is
responsible for active transport of a new polyketide. In my thesis I clearly proved that this protein is necessary for active efflux of the polyketide. Using the PCR targeting method I constructed S. coelicolor cpkF deletion mutants, and then complemented them by introducing cpkF gene in another site on the S. coelicolor chromosome. I constructed overproduction mutant by introducing additional copy of the cpkF gene on an integration vector to S. coelicolor wild strain. I also prepared constructs for heterologus expression of CpkF protein in E. coli and scanned cultivation conditions for protein isolation.
Responsibilities:
Digestion of plasmid DNA with restriction enzymes,
PCR, PCR targeting.
Isolation of plasmid DNA ,
ligation of isolated fragments, transformation,
Gelelectrophoretic analysis,
Work in sterile conditions, media preparation, buffers, reagents preparation,
Proteins expression,
Sonication,
SDS – page,
Immunoblotting.
Supervisor: dr. Krzysztof Pawlik
Biomedical Institute of University of Antwerp, Belgium
. – . Research Project – ‘’Cytoglobin and Neuroglobin, their expression in E. coli
and purification’’,
The aim of this project was to overexpress Neuroglobin and Cytoglobin in E. coli using pET vector system to get appropriate volume of proteins which will be useful for investigation their function, structure and interactions. And because we can not avoid of expression any other contaminent protein in bacteria I had to purified my globins using standard protein purification steps.
Responsibilities:
Globins expression,
Isolation and purification of proteins: sonication, ammonium sulfate precipitation and dialysis, ion exchange chromatography, concentration, gel filtration,
SDS – page,
Sample preparation, media preparation, buffers, reagent and antibiotics solutions preparation, autoclaving, sterility.
Polish Academy of Sciences
Institute of Immunology and Experimental Therapy, Wroclaw, Poland
. – . Biotechnology project – ‘’ Characterization of the production
conditions of the Streptomyces coelicolor Cpk polyketide synthase product’’, Responsibilities:
Digestion of plasmid DNA with restriction enzymes,
Isolation of plasmid DNA,
Ligation of isolated fragments, transformation,
Gelelectrophoretic analysis,
Work in sterile conditions,
Media, buffer reagents and solutions preparation.
Highschool: V Liceum Ogólnokszta&#;c&#;ce in Bielsko Bia&#;a
Profile: mathematics–physics–computer science
Hospira Ltd.
.. – .. Work position – Technician assistant
Responsibilities:
Maintain stock of cleanroom clothing,
Replenish stock the preparation room on daily basis,
Rotation stock where necessary,
Carry out monthly stock checks,
Prepare documentation and assemble raw materials ready for compounding of sterile products,
Desinfection of critical areas, preparation areas and its equipment,
Compound of sterile products.
Soap and Co., UK, London
.. – .. Sales assistant
responsibilities: presenting and selling cosmetics
Herbalife, Pl, Wroclaw
. – . Personal advisor - nutritionist
Pl, Wroclaw
. – . Babysitter
Hard Rock Café, UK, London
. – . Waitress
responsibilities: serving
Work and Travel, USA, Washington DC
. – . Restaurant hostess and receptionist – Hotel Washington,
responsibilities: greeting quests, taking reservations
. – . Waitress – Listronis Restaurant,
responsibilities: serving
Work and Travel, USA, New Orleans, Washington DC
. Office help – PWC, Washington
. – . Park hostess – Six Flags, New Orleans
responsibilities: game attendant
Mostra Bar and Grill, Greece, Paralia
. – .. Waitress
responsibilities: serving, preparing food
Karel de Grote–Hogeschool, Antwerp, Belgium
.. – . Communication and Management for Engineers Course–
‘’Belgium culture and economy’’,
’’Presentation skills’’
Krystyna &#;&#;czy&#;ska Academy of Beauty, Krakow, Poland
.. – .. Art- Visage and Styling Course
Main topics:
- visagiste work stand and accessories;
- beauty perfections – typization and individualization ;
- characteristic features and face shape;
- colours theory and harmony;
- fashion style corection, main colours, their composition and
combination;
- technics of profesional make-up practice;
- camouflage
- modeller
- face shape corection;
- stylization – hair, clothes
- face visage.
Hospira Aseptic Services Ltd. London, UK
.. – .. Good Manufacturing Practices, Good Documentation Practices, Good
Laboratory Practices Courses
English advanced
German basic
Spanish basic
Polish native
Microsoft Office (Excel, Outlook, Word, Power Point), CorelDrw, Cloning Manager, Artemis, GeneDoc, PhotoShop
Interests Cycling, swimming, jogging, playing tennis, travels, natural science, popular psychology,
Are available upon request
