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Mrs. S . Marakadambal W/o.N. Suresh Kumar, / L Kamaraj Nagar, Sirumugai – . Coimbatore (Dt) INDIA. E-mail: Mobile NO: . Objective: Seeking suitable or any position aspires to work with an organization where I would be able to utilize my skills and also hone them to face greater challenges. Summary: • Experience in plant tissue culture technique under aseptic condition in clean room environment (eg, micro propagation, media preparation etc.,) • Have good knowledge of procedures used in plant biotech laboratory. • Have experience in nursery plantation and management. • Excellent organizational & inter-personal skills. • Good communication skills and team work capabilities. • Ability and motivation to learn new technologies. • Good communication and interpersonal skills . • Able to perform task assigned independently and expeditiously. • Willingness to learn, highly committed and prepare to overtime is a prerequisite. • High level of accuracy and integrity while working under pressure. • Proactive and versatile team player. • Strong analytical skills with positive attitude and pleasant personality. • + Years of experience in R & D and Commercial Plant Tissue Culture Laboratory. • Specialized in Initiation Technology, R&D, Protocol Developments. • Good knowledge in Media formulation and Preparation. • Hand on experience in Contamination Control Systems, Production and varietals improvement programs. ( Breeding , Physical treatments and chemical treatments) • Having good technical knowledge in any type of plants propagation. • Having adequate technical knowledge in organic solid and liquid fertilizer production and organic firming. • Having adequate technical knowledge of indigenous plant growth nutrients production. • + years of working experience in rose and orchid floriculture unit. Practical (Hands-On) Experience: • Main function in performing plant micro propagation using aseptic techniques in clean room. • Select high quality mother plant or live material with the desired characteristics. • Cut, sterilize, test for viruses and other diseases, and prepare selected tissues for propagation. • To support in vitro plant culture work, involving media preparation, and maintaining a culture collection. • Preparing schedules for the micro propagation with consideration of numbers of plants required, stocks on hand, work priorities and others relevant factors. • Ensure the continuous production of plantlets. • Perform daily replacing of cultured plant material onto fresh media under sterile conditions. • Monitors and records daily plant production and prepares weekly and monthly reports. • Preparation of stock solutions for culture media. • Implementation of new protocols to bring new plants into production within special growth media. • Maintains a high degree of sterile working conditions to reduce the occurrence of contaminated media / plantlets. • Plan, organize and execute experimental works as discussed by the research team. • Record all observations, data and results. • Assist in analyzing the results. • Upkeep and maintain the Lab records. • Prepare technical reports, summaries, protocols, and quantitative analysis. • Provide support for the implementation of Good Laboratory Practices. • Assist in the order & maintenance of lab supplies. • Assist in setting up equipment. • Clean, sterilize and maintain equipment and facilities. • Prepare lab reagents and media. • Maintain familiarity with current scientific literature and contributes to the process of a project within his or her scientific discipline. • To support routine work carried out in the general laboratory and to support and supervise staffs. • To support molecular systematic work involving plants. • Undertake emergency work as and when required. • Ensures work is completed to Departmental standards. • Assist in some administration work. • Record : date culture flasks are replaced, usage of alcohol and disinfectants, fertilizer and chemical registers, asset inventory / stock controls, uniforms and protected clothing Issues and other necessary records. • Prepare nursery records. • Ensures that all staffs adhere to all safety requirements allied to the operation. • Conduct routine safety inspections of all plant, machinery equipment and buildings reporting all breakages / problems to the Tissue Culture Technician. • Perform all relevant work / activities assigned by superiers. Career Experience: Tropical bio sciences, pala, kerala, India (Contract starts from : August ) Designation : Production manager Responsibilities: Laboratory design, setup and execution of million plants (Grand nine, Orchid, Anthurium, Gerbera) per annum capacity lab. Media formulation and preparation. Standardization of initiation technology and culture quality maintenance. Cost effective process technology media formulation and laboratory management. Process chart designing, production plan, constant production design and production forecasting. Development of Hardening Technique. Laboratory management like R & D, Production and Administration. Nursery practices and Marketing strategy development. Achievement: With in months from commencement of the new lab, monthly around , hardened banana plantlets being produced from clumps of imported mother tissues from Israel through just laminar seats capacity at the rate of hours/shifts/days per month. Especially only % mortality in hardening of plant. Novel plants sdn bhd, Malaysia. (Contract start: March Contract end: July ) Designation: Assistant production manager (Jatropha(bio-diesel) plant tissue culture research project) Responsibilities: Laboratory design, setup R&D lab for jatropha research project. Media formulation and preparation. Standardization of initiation technology and culture quality maintenance. Cost effective process technology media formulation and laboratory management. Development of Hardening Technique. Laboratory management like R & D, Production and Administration. Managing the jatropha seedlings nursery. Floriculture: pochiraju industries limited near bangalore, India. (Contract start: Sep Contract end: Feb ) Designation: Production executive Responsibilities: Managing rose and orchid floriculture unit work force. Implementing own preparation of plant fertilizer method for floriculture farming. Implementing organic farming in rose floriculture. Large-scale production of verities of rose cut flower and verities of orchid cut flower. Involving trials of new farming techniques. Elimination/control of plant dieses. Planning of purchasing farm requirements. Propagation of rose & orchid plants. Nursery practice development (hardening of plants) Sri Krishna Biotech Plant Tissue Culture Unit Sirumugai, India (Contract Start: Dec Contract End: Aug ) Designation: Working Partner. Responsibilities: Laboratory design, setup and execution of million plants / annum (Grandnine, Vanilla) capacity lab. System development of some banana plant varieties through Tissue Culture and some varieties under R & D. Media formulation and preparation. Standardization of initiation technology and culture quality maintenance. Cost effective process technology media formulation and laboratory management. Process chart designing, production plan, constant production design and production forecasting. Development of Hardening Technique. Laboratory management like R & D, Production and Administration. Nursery practices and Marketing strategy development. Developed tissue culture technology and large scale production of tree species like, Eucalyptus, Neem, Accacia, Mangium, Teak etc. . Exhibitions participated: Exhibited Vanilla Tissue culture germ plasms and plants at “Agri Intex and ” Coimbatore, Tamilnadu, India. Exhibited Jatropha carcus germ plasms and Tissue culture plants at “ Agri Intex ” Coimbatore, Tamilnadu, India. Achievement: At the period of working as a working partner at Sri Krishna Bio-Tech, the U.K based D bio oils Co. has given order for one million plants / year and advance amount Rupees Lakes due to my research work and standardization of jatropha carcus. (Bio-diesel project) Dalmia center for Biotechnology Coimbatore, India (Lab production capacity-.b million) (Contract Start: Feb Contract End: Aug ) Designation: Scientific Technical Assistant Responsibilities / Achievements Laboratory design, setup and execution of . million plants / annum capacity lab. Preparation of Plant Tissue Culture Media Selection of Plant Material and Explants initiation Standardization of explants type and sterilization Technology. Large scale tissue culture plant production of the following plant species: Orchid, Neem. Laminar flow work in Plant Inoculation room and plant growth room. Initiation and process ratio trials for new products. Elimination / control of plants phenolics and systemic bacteria during initiation. Breeding and hybrid seed production in Anthurium. Implementing hardening methods for lab TC Plants in green house. SIV Industries Ltd., Sirumugai, India (TC lab setup of . million capacities and production of ornamentals and plantation crops for domestic and Vanilla project for Spices Board Cochin (Govt. of India under taking)) (Contract Start: Oct Contract End: Dec ) Designation: Scientific Technical Assistant (Contract restart: Jan Contract End: Oct ) Designation: Production Contractor – Tissue culture Responsibilities: Preparation of Plant Tissue Culture Media Selection of Plant Material and Explants initiation Standardization of explants type and sterilization Technology. Large scale tissue culture plant production of the following plant species: Banana, Sugarcane, Vanilla, Carnation Laminar flow work in Plant Inoculation room and plant growth room. Initiation and process ratio trials for new products. Elimination / control of plants phenolics and systemic bacteria during initiation. Personally involved in technical training given for all section technicians. Laboratory management like R& D, Production and administration. Implementing hardening methods for lab TC Plants in green house AVT Biotechnology, R & D Division, Kerala, India (Contract Start: Jan Contract End: Aug ) Designation: Operator Responsibilities: Preparation of Plant Tissue Culture Media Selection of Plant Material and Explanted initiation Standardization of explants type and sterilization Technology. Large scale tissue culture plant production of the following plant species: Anthurium, Aconitum, Alstremaria, Alpinia Banana, Black pepper, Coffee, Cardamom Cymbidium, Cord line, Dieffenbachia, Phalanoepsis Papaya, Syngonium, Spathiphyllum, Tea Teak, Philodendron, Pelargonium, Vanilla, Rubber Turmeric, Dendrobium orchids, Dianthus Date palm, Ficus elastica, Ficus benjamina Freesia, Gerbera, Hellebores, Lily Pionea, Pavonia and etc Undergone Laminar flow work in Plant Inoculation room and Plant growth room. Initiation and process ratio trials for new products. Elimination / control of plants phenolics and systemic bacteria during initiation. Breeding and hybrid seed production in Anthurium. Implementing hardening methods for lab TC Plants in green house. Short Note on Plants worked and expertise: • Alocasia Sandereana Bullet – Micro propagation • Ficus elastica Green - Robusta Black - Black prince Variegata - Tinake - Micro propagation • Ficus benjamina Golden King, Exotica, Minilucy, Star light, Reginald, Foliole Prestige - Micro propagation • Dieffenbachia Camilla Ammoena - Micro propagation • Alpinia Variegated ginger Purpurea - Micro propagation • Pelargonium Pelargonium goaveolance (Mosquito repellent plant) • Syngonium Red butterfly, White butterfly, Lemon Lime, Light green Andrea - Micro propagation • Gerbera Red, Yellow, Crimson, Pink, Orange, White, Pink, Rose, Lemon yellow, Violet Pot varieties. – Micro propagation • Calathea Open Himiana, Rosea picta Picturata - Micro propagation • Spathyphyllum Pettile Viscount – Micro propagation. • Orchids D.Sonia , Rinappa, Banyat pink, New wannie Sonia- - Micro propagation • Phalanoepsis • Cymbidium - Micro propagation • Philodendron Salome, Xanadu, Black prince, Pluto Imperial Red - Micro propagation • Anthurium Red, Orange, White, Pink, Agni hotri Deep red - Micro propagation • Cord line Austalis • Carnation • Xanthosoma Lindenii – Micro propagation • Caladium • Lily • Vanilla • Marigold - Selection from Mexico (Tagetus) for food color extraction • Teak - Selected varieties • Eucalyptus – selected varieties form SIV • Cord line Australis Atro • Cord line Red Star • Cord line Sundance Education: Pre-degree Course Calicut University, Kerala, India. (Year completed: )