Fresher M.SC -Biochemsitry Resume

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Posted on: 2010-02-01

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Resume:

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Education Qualification:

S.No.	Qualification	School/College Name	Percentage Secured
.	M.SC  Biochemistry	GITAM College, Visakhapatanam.	. (CGPA)
.	Degree ( Microbiology, Biochemistry, Chemistry)	CH.S.D.St.Theresa’s College, Eluru.	%
.	Intermediate(Bi.P.C)	CH.S.D.St.Theresa’s College, Eluru.	%
.	S.S.C	St.Theresa’s High School,Eluru.	%

 

Computer Proficiency:

 

• MS DOS.


• Microsoft Office.


• Windows XP, .

 

Achievements:

 

• Secured state ^rd rank in AUCET


• I stood topper in B.Sc with an aggregate of %.


• Stood topper in M.Sc with an aggregate of . from GITAM University

 

Theoretical Knowledge:

 

• HPLC, GC.


• U.V- Visible Spectophntometry.


• I.R Spectrophotometry.

 

RESEARCH (PROJECT) DONE

 

 

Research Title:  Biochemical Assays for Haemophilus influenzae type B Conjugate Vaccine

 

Place                : R&D, Bharat Biotech International Limited, Hyderabad.

Duration         :   Months. 

Description     :

                           Haemophilus Influenzae type b is a small, non motile, gram negative coccobacillus. In the present work I have analyzed the components of the PRP-T conjugate vaccine by several biochemical and analytical assays. PRP-T Conjugate vaccine comprises of PRP (Polyribosyl Ribitol Phosphate) and tetanus toxoid carrier protein. To check the purity of vaccine the amount of free PRP and protein are estimated at different manufacturing stages. The protein content in the bulk, conjugate and filled vials can be estimated by Lowry’s method, modified Lowry’s method and BCA method. The PRP content can be estimated by Orcinol method. The PRP and protein components were found to be within the normal range. LPS (Lipopolysaccharide) is an impurity in conjugate vaccine. I have done modified silver staining technique using SDS-PAGE Electrophoresis to detect LPS. Impurities can also be removed by K Nanocep centrifugal device. Hence by performing different biochemical assays we can check the purity of vaccine.  

 

Equipment Handled:

 

• Elecrophoretic Apparatus.(SDS-PAGE)


• Centrifuge


• U.V- Visible Spectrophotometer

 

 

Practical skills:

 

• Isolation of RNA and DNA.


• Agarose gel electrophoresis, SDS-Poly Acryl amide gel electrophoresis.


• Chromatographic techniques.(Paper, TLC, Column, Ion exchange)


• Southern blotting.


• Plant tissue culture.

 

Seminars Delivered:

 

• Antigen processing and presentation.


• About Monoclonal antibodies.


• Use of drug designing in Bioinformatics.