Assitant Prof Resume
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Resume:
Seeking a challenging assignment as…
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Scientist / Project Manager / Senior Research Associate / Consultant / Expert
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target sector: Pharmaceutical / Life Sciences / Biotechnology Academic
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A highly qualified Biologist with over years of professional and research experience ( years in USA and year in Taiwan and presently as a Assitant Prof in Biotechnology in ETHIOPIA) with expertise in molecular biology, plant biotechnology, tissue culture and animal cell culture technology, project management and experience in teaching at Universities in India and the US.
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Educational Qualifications
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- PhD (Biotechnology) with specialization in plant tissue culture, biotechnology & molecular biology from Osmania University in (with CSIR, Council of Industrial and Scientific Research Senior Research Fellowship).
- M Sc. (Botany) with specialization in cytogenetics from Osmania University in
- B Ed. (Biology) with specialization in Biology & English from Osmania University in .
- B Sc. (Botany, Zoology & Chemistry) from Osmania University in
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Trainings & Workshops Attended
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- EMBO Practical Course on Plant Reproduction: selected for training in Beijing, China in Aug Sep .(one among selected).
- Plant Development Arabidopsis Model System: International Workshop held at National Center for Biological Sciences, Indian Institute of Science, Bangalore, India in Dec .
- Cyanobacterial DNA Techniques: a Workshop at the Department of Biotechnology, Center for BGA Biofertilizer, Madurai Kamaraj University, Madurai, India in Mar .
- Recombinant DNA Techniques: an Internal Workshop at Madurai Kamaraj University, Madurai, India, sponsored by Scigenics, Madras and Life Technologies Inc., USA in Oct .
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Technical Expertise
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- Skilled in conducting research and techniques in biotechnology.
- Highly analytical with ability to learn and use new skills / techniques quickly.
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DNA:
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- Isolation of genomic DNA from bacterial, yeast, plant and mammalian cells.
- Extraction and purification of plasmid DNA.
- Retrieval of DNA by cloning, sequencing, southern analysis, PCR – single cell PCR (bacteria & yeast), tissue PCR in plants and other PCR techniques.
- DNA synthesis and BrDu labeling in cell cultures.
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Transformation:
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- Bacterial & Yeast: Cacl and electroporation, agro bacterial transformation in plant systems, transfection in cell cultures.
- RNA:
- Isolation from bacteria, yeast, plant and mammalian cells by: northern blotting and dot blot, RT-PCR, gene expression by micro array methods.
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Promoter:
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- Promoter and Lac-Z-Fusions (yeast genes)
- Promoter replacement by Tet-O-Promoter systems
- Linear integration of genes / promoters / plasmids into yeast genome by homologous recombination.
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Complementation Test: on yeast & bacteria.
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Protein:
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- Extraction from bacteria, yeast, plants and mammalian cells by western blotting, immunoprecipitation, ELISA, immunohistochemistry, agglutination test, electrophoresis, immunodiffusion.
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Assays:
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- Using spectrophotometer, fluorimetry, luminometry and isotopic methods, MTT assay, &#;-gal assay in yeast system, GC sterol profile, citrate snthase enzyme assay.
- Cell & Tissue Culture:
- Bacterial, yeast, plant and mammalian cell cultures (Human & rat smooth muscle cells, Hela cells, Hep cells). Freezing and stocks preparations.
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Chlamydial Culture:
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- Chlamydial culture, titer, infection & harvest.
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Microscopy:
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- Phase contrast microscopy, SEM, immunofluorescence, FACS analysis, confocal microscopy.
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Others:
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- Construction of binary vectors for plant systems.
- Screening cDNA library, ’RACE, density centrifugation & database search.
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Scientific Talks : Presented scientific talk shows on biotechnology in YuvaVani (All India Radio) - shows from to
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Computer Skills:
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- Microsoft Office (Word, Excel, PowerPoint), Graphics and Imaging: Adobe Photoshop, C, C++, Oracle, UNIX Languages, Scanning, Database Searches and Internet Searches.
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Strengths
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- Responsible and efficient in laboratory organization and administration.
- Punctual and strive to maintain time schedules.
- Excellent interpersonal, communication, presentation and leadership qualities.
- Ability to easily mingle with people of diverse cultures as also subordinates, peers and seniors.
- Good orator with ability to impress and convince others.
- Strong in problem solving and can work well independently as well as in a team.
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Professional / Research Experience
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Present Position
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Assitant Prof Biotechnology
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Applied science
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Department of Biotechnology
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University of GONDAR
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ETHIOPIA TILL DATE Under UNDP – United nations developing ProgramME.
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Prof GM Reddy Research Foundation R&D, Hyderabad
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Scientist
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Working on a project entitled ‘Survey, collection and nutritional assessment of lesser known edible plant resources used by major tribes of Andhra Pradesh, India) as a Scientist and Principle Investigator.
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Department of Surgery, College of Medicine, University of Tennessee Health Center, USA
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Guest Scientist)
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Worked on a project entitled ‘Ca/muscular dystrophy and cardiomayapathi in hamsters’ - Analysis of Zn / DMS / Cardiomyopathy on Hamsters
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Department of Molecular Sciences, University of Tennessee Health Center, USA
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Post Doctoral Fellow
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Worked on culture, infection and harvest of Chlamydia Sp.
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Department of Physiology & Biophysics, University of Tennessee Health Science Center, USA
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Post Doctoral Fellow
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Worked on vascular remodeling with cPLA (cytosolic phospholipase A) in human and rat smooth muscle cells. Successfully created a new double mutant phenotype by disrupting the Erg gene for the ncr mutant, which is responsible for Niemann Pick disease in humans. In yeast, the function of this gene is not known. However, there are speculations that this gene might be involved in sterol trafficking, as confirmed by GC and GC-Mass Spectrometer. Also developed a double mutant phenotype for the arv gene (novel gene), which is a mediator of sterol trafficking with a functional human homolog.
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Department of Biology, Indiana University / Purdue University at Indianapolis, Indiana, USA
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Post Doctoral Research Associate
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Worked on transcriptional regulation of C- demethylation genes in Saccharomyces and model studies on ncr and arv genes in Saccharomyces. Studied the roles of Erg , and genes in the removal of two methyl groups at C- of the sterol molecule of sterol biosynthetic pathway in saccharomyces cervisiae. The demethylation at the C- position appears to be the sole target of azole antifungal activities. The main objective was to analyze the C- demethylase genes in Saccharomyces to determine the regulatory mechanisms governing these genes and to assess the possibilities of their suppression which may contribute to their resistance to antifungal agents. Achieved a very good &#;-gal activity for the Erg & promoters fused to Lac Z. Further planned study the interactions between Erg , and with Erg gene at the transcriptional level.
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Institute of Botany, Academia Sinica, Taiwan
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Post Doctoral Research Associate
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Worked on sucrose synthase gene isolation and characterization in Arabidopsis thaliana, a plant model system. This enzyme is one of the key enzymes responsible for sucrose and carbohydrate metabolism in higher plants. Only genes had been reported previously. Identified genes, including the reported in Arabidopsis. Successfully cloned full-length cDNAs and confirmed for intron splicing and mutations by sequencing the clones.
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Center for Cellular & Molecular Biology, Hyderabad, India.
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Post Doctoral Fellow
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Worked on isolation, cloning and over expression of citrate synthase gene in Arabiodopsis thaliana. The over expression of enzyme citrate synthase leads to uptake of phosphate ions via roots from soil, the excess enzyme emerging from roots acts as a chelating agent. The candidate gene was successfully isolated, sequenced and cloned into a binary vector for plant transformation. Standardized the enzyme assay for this gene. Screened Arabidopsis plants for female gametophytic mutants using transposon mutagenesized lines of Arabidopsis by gene trap and enhancer trap lines.
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PhD Dissertation
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Title: ‘Induction of haploidy by anther culture and microspore culture in Pigeon pea (Cajanus Cajan (L) Mill Sp.)’ a grain legume of tropics and subtropics.
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Research focused on haploidy: anther and microspore (which provide excellent experimental system for genetic transformation, mutant cell selection, haploid breeding, study of embryo development at the cellular, molecular & biochemical levels) cultures were employed for the production of haploids from pollen. Haploid chromosome number was confirmed by various experimental methodologies. Factors for callus induction, somatic embryogenesis and regeneration studies from different explants of cultivars and mutants were standardized.
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Teaching Experience
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Presently teaching at Ethiopia Gondar – Biochemistry and Biotechnology and Molecular biology for Graduate students. TILL DATE
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- Molecular biology at the Undergraduate and Graduate levels at the Department of Biology, Indiana University-Purdue University at Indianapolis, IN, during November .
- Theoretical and practical plant biotechnology at the Masters Degree level at the Department of Botany, Osmania University, Hyderabad, India - July to Feb .
- Genetics course, both theoretical and laboratory sections at the Undergraduate level at the Department of Botany, Nizam College, Osmania University, Hyderabad, India - During academic year .
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Publications
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. Virupapuram Vijaybhaskar; Veeraputhiran Subbiah; Jagreet Kaur; Pagadala Vijaya Kumari; Imaran Siddiqi
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Identification of a Root-Specific Glycosyltransferase from Arabidopsis and Characterization of its
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Promoter - Journal of Biosciences – Vol.,(),-,.
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- P Vijaya Kumari: ‘Direct somatic embryogenesis from mature leaves of pigeon pea [Cajanus Cajan L. Mill Sp.]’ - Plant Molecular Biology and Biotechnology, .
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. P.Vijaya Kumari, Survey collection and nutritional assessment of lesser known adible plant resources used by major tribes of Andhra Pradesh. DBT’s Annual Report letters(-). (Revision).
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. Siddiqi I, G Ganesh, J Kaur, V Subbiah and P Vijaya Kumari: ‘Information and tools from Arabidopsis for biotechnology of crop plants in sorghum tissue culture’ - Transformation and Genetic Engineering, Eds N Seetharama and I D Godwin, Science Publishers Ins., New Hampshire, USA. ISBN - - - – , .
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- Vijaya Kumari P, B Kavi Kishore and J K Bhalla: ‘In vitro plant regeneration in pigeon pea [Cajanus Cajan (L.) Mill Sp.] Via organogenesis’ - Plant Cell Biotechnology and Molecular Biology, (&): -, .
- P Vijaya Kumari and S Narasimha Chary: ‘Induction of embryogenesis by anther culture of pigeon pea’ - In vitro Cellular and Developmental Biology, Vol A, A, at the Congress on Cell and Tissue Culture held in Denver, Colorado, USA in May .
- P Vijaya Kumari and S Narasimha Chary: ‘Anther culture studies in pigeon pea’ - In vitro Cellular and Developmental Biology, Vol. A, , at the Congress on Cell and Tissue Culture held in Columbia, Missouri in Jun .
- P Vijaya Kumari and S Narasimha Chary: ‘Induction of haploidy by anther culture and isolated microspore cultures of pigeon pea’ - VII International Congress of Plant Tissue and Cell Culture in Firenze, Italy in Jun .
- S Narasimha Chary and P Vijaya Kumari: ‘Somatic embryogenesis and plant regeneration from leaf explant of Cajanas Cajan (L) Mill SP’ - VII International Congress of Plant Tissue and Cell Culture in Firenze, Italy in Jun .
- P Vijaya Kumari and S Narasimha Chary: ‘In Vitro plant regeneration through organogenesis in pigeon pea’ - In vitro Cellular and Animal Developmental Biology, Vol. A, A, . Research Triangle Park, North Carolina.
- P Vijay Kumari, S Narasimha Chary and J K Bhalla: ‘Organogenesis and somatic embryogenesis in callus cultures of induced mutants of pigeon pea’ - In vitro Cellular and Animal Developmental Biology, Vol. A, A, . Research Triangle Park, North Carolina.
- P Vijaya Kumari and S Narasimha Chary: ‘Embryogenesis from isolated microspores of pigeon pea’ - In vitro Cellular and Animal Developmental Biology. Vol. A, A, . Research Triangle Park, North Carolina.
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OTHER ACTIVITIES
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One of the best Volunteer to help the ORPHAN CHILDREN of WORLD VISION, MOTHER THERESA CHARITY and involved in BLOOD DONATION camps.
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Dr. Pagadala Vijaya Kumari.
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