MICROBIOLOGIST Resume
CURRICULUM VITAE
Donthireddy Madhavi
Tellicherrycourt,
Jerseycity,Newjersey,
Email;
Ph no;,
EDUCATIONAL BACKGROUND;
Masters degree in microbiology with % at srivenkateswara university during -.
B.sc degree(microbiology,biochemistry,chemistry) with % at srivenkateswara university
During-.
Intermediate(+) with % at KORA Junior college(Nellore) during .
EXPERIENCE;six months work experience in an hospital lab.
LABORATORY SKILLS:
GENERAL MICROBIOLOGY & BACTERIOLOGY:
Microscopic observation of microorganisms by different staining procedures, Pure culture techniques, Isolation & Propagation of microorganisms, Biochemical identification of bacteria.
BIOLOGICAL CHEMISTRY:
Qualitative tests for Carbohydrates, Aminoacids & nucleic acids, Quantitative estimation of protein, glucose, glycine, cholesterol, bilirubin & inorganc phosphate, Determination of activity of peroxidase & polyphenol oxidase.
ANALYTICAL TECHNIQUES:
Agarose gel electrophoresis, SDS PAGE, Paper & Thin layer chromatography, Membrane dialysis, Isolation of chloroplast, estimation of chlorophylls, Determination of lmax values for coloured solutions.
MICROBIAL PHYSIOLOGY & GENETICS:
Bacterial growth curve, Effect of temperature, PH ,salt on bacterial growth, Isolation of spore forming, antibiotic resistant, U.V. resistant bacteria. Isolation of Chromosomal & Plasmid DNA, Observation of mitosis & meiosis.
MEDICAL MICROBIOLOGY:
Isolation of Microbial flora of skin, saliva, throat, Anti microbial sensitivity test, WIDAL test, Latex agglutination test for HBV, Examination of blood for Malarial parasite, Diagnostic tests (HIV, RA, Syphilis).
IMMUNOLOGY:
Total count of blood cells, Differential count of WBC, Haemoglobin estimation, Blood typing & Rh determination, Purification of immunoglobulins, production of polyclonal antibodies, Invitro serological techniques (single & double immunodiffusion tests, Immuno,counter current, rocket & radial electrophoresis, HA & HI tests, ELISA ).
MOLECULAR BIOLOGY & rDNA TECHNOLOGY:
Isolation of plasmid DNA from E.coli, isolation of total RNA from dried yeast, Recovery of DNA from agarose gels, Quantitative estimation of Nucleic acids, Southern & Western blotting, Dot blot.
BIOSTATISTICS:
Construction of a histogram,Uncertainities in the estimation of means and variances-t,F,Z tests,Chi-squaretest,Statistical treatment to count data:Poisson distribution,standard error,confidencelimits of counts.Regression formulae and application,Fitting the best straight line through a series of points,Fitting of different curves,Statistical basis of biological assays:Response-dose-metameter,Delusion assays,Direct and indirect assays,Quantal responses,LD,PD,ED parallel assay,slope ratio assay.
BIOINFORMATICS:
Virtual Library-:searching MEDLINE;pubmed.Virtual Library- Science CitationIndex and current awareness services,
Retrival of sequences in FASTA format, BLAST search, Secondary structure prediction, Viewing PDB files using RASMOL, use of CLUSTAL X, Tree View & DNASIS softwares.
One year certificate course in MS OFFICE.
MICROBIOLOGICAL SPECIALIZATIONS:
Different Fermentation techniques, Isolation of different microorganisms from Soil, Water, Milk & Food Products, Determination of metabolic activity of microorganisms in the soil.
