Post doctoral fellow Resume
Angelique BIANCOTTO
Indian Hills terr
Derwood MD
Professional address
LCMB/NICHD/NIH
, center drive
Bldg room D
Bethesda MD
Tel
Fax
Email
UNIVERSITY EDUCATION
• : Ph. D Specialty Immunology:
“HIV- persistence in AIDS”
University Aix-Marseille II, in the INSERM U in Marseille Luminy under supervision of Dr. Ivan Hirsch
• : M.S. Immunology
(French Diplome d’Etudes Approfondies en Immunologie)
(September to September ), University of Aix-Marseille II, in the INSERM U in Marseille Luminy under supervision of Dr. Ivan Hirsch
• : B. S. Biochemistry and molecular biology
(French Maitrise de Biochimie mention biologie moleculaire),
Faculte des Sciences de Luminy, Universite Aix-Marseille II.
Major in Immunology.
RESEARCH EXPERIENCE
September - present, Post-doctoral fellow: Study of coinfection with HIV- and different pathogens in an ex vivo model. Study of immune dysregulation upon HIV- infection. LCMB, NIH. Bethesda
February - March , Post-doctoral fellow: Preparation of a substractive library from cDNA of non-infectious HIV infected tonsils histocultures. St George’s hospital medical school London.
July - July , Ph.D. student: Characterization of latently infected T lymphocytes in an in vitro model developed in our laboratory. Analysis of the phenotype of resting memory T CD+ cells in an ex vivo model: tonsils tissue. Utilization of HIV derived vector. INSERM U-Marseilles
August - July , Graduate student: Characterization of replication of different HIV- derived virus in T CD+ cells. INSERM U-Marseilles
December - January , Graduate student: Characterization of thymic cells expressing Vanin-.
TECHNICAL EXPERTISE
Cell Biology Cell cultures: Maintenance of human cell lines (adherent and in suspension), primary cultures of PBMC, cellular cloning. Cell separation with magnetics beads.
Flow cytometry FACS (Calibur, Quanto, LSR II). Cell separation (Aria),
Multiplex bead array Development and validation of Luminex based (Luminex, Biorad)
Retrovirology Viral stocks preparation, infection, transfection, titration, RT activity, ELISA p.
Molecular Biology DNA analysis: cloning, sequencing, sequence analysis, RT-PCR, semi-quantitative RT-PCR, Real time PCR, Southern blots, Northern blots. Substractive library of cDNA.
Immunochemical analysis Nuclear and cytoplasmic's proteins separation, western blots.
Immunohistochemistry Mouse dissection. Thymus extraction. T lymphocytes isolation. Tonsils, lymph nodes, rectal biopsies, cervix dissection, D histocultures.
ORIGINAL CONTRIBUTIONS IN PEER REVIEWD JOURNALS
. Naspetti, M., Martin, F., Biancotto, A., Malergue, F., Mansuelle, P., Galland, F., and Naquet, P. . A novel anti-Ep-CAM antibody to analyze the organization of thymic medulla in autoimmunity. Curr Top Microbiol Immunol :-.
. Gondois-Rey, F., Biancotto, A., Pion, M., Chenine, A.L., Gluschankof, P., Horejsi, V., Tamalet, C., Vigne, R., and Hirsch, I. . Production of HIV- by resting memory T lymphocytes. Aids :.
. Gondois-Rey, F., Grivel, J.C., Biancotto, A., Pion, M., Vigne, R., Margolis, L.B., and Hirsch, I. . Segregation of R and X HIV- variants to memory T cell subsets differentially expressing CDL in ex vivo infected human lymphoid tissue. Aids :.
. Grivel, J.C., Biancotto, A., Ito, Y., Lima, R.G., and Margolis, L.B. . Bystander CD+ T lymphocytes survive in HIV-infected human lymphoid tissue. AIDS Res Hum Retroviruses :-.
. Pion, M., Jordan, A., Biancotto, A., Dequiedt, F., Gondois-Rey, F., Rondeau, S., Vigne, R., Hejnar, J., Verdin, E., and Hirsch, I. . Transcriptional suppression of in vitro-integrated human immunodeficiency virus type does not correlate with proviral DNA methylation. J Virol :-.
. Biancotto, A., Grivel, J.C., Gondois-Rey, F., Bettendroffer, L., Vigne, R., Brown, S., Margolis, L.B., and Hirsch, I. . Dual role of prostratin in inhibition of infection and reactivation of human immunodeficiency virus from latency in primary blood lymphocytes and lymphoid tissue. J Virol :.
. Gondois-Rey, F., Biancotto, A., Fernandez, M.A., Bettendroffer, L., Blazkova, J., Trejbalova, K., Pion, M., and Hirsch, I. . R variants of human immunodeficiency virus type preferentially infect CDL- CD+ T cells and are potentially resistant to nucleoside reverse transcriptase inhibitors. J Virol :-.
. Lisco, A., Grivel, J.C., Biancotto, A., Vanpouille, C., Origgi, F., Malnati, M.S., Schols, D., Lusso, P., and Margolis, L.B. . Viral Interactions in Human Lymphoid Tissue: HHV- Suppresses the Replication of CCR-tropic HIV- via CD Modulation. J Virol.
. Biancotto, A., Grivel, J.C., Iglehart, S.J., Vanpouille, C., Lisco, A., Sieg, S.F., Debernardo, R., Garate, K., Rodriguez, B., Margolis, L.B., et al. . Abnormal activation and cytokine spectra in lymph nodes of persons chronically infected with HIV-. Blood.
. Grivel, J.C., Elliott, J., Lisco, A., Biancotto, A., Condack, C., Shattock, R.J., McGowan, I., Margolis, L., and Anton, P. . HIV- pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR- and CXCR-tropic HIV-. Aids :.
. Lusso, P., Crowley, R.W., Malnati, M.S., Di Serio, C., Ponzoni, M., Biancotto, A., Markham, P.D., and Gallo, R.C. . Human herpesvirus A accelerates AIDS progression in macaques. Proc Natl Acad Sci U S A :-.
. Vanpouille, C., Biancotto, A., Lisco, A., and Brichacek. . Interactions between HIV- and vaccinia virus in human lymphoid tissue &#;ex vivo. (J. Virol, in press)
. Biancotto A., Iglehart SJ., Vanpouille C., Condack C.E., Lisco A., Ruecker E., Hirsch I., Margolis L. and Grivel JC. HIV- production and depletion of activated and non-activated cells in human lymphoid tissue ex vivo (Blood in press)
ARTICLES IN REVISION OR PREPARATION
. Biancotto A., Iglehart SJ., Lisco A., Vanpouille C., Grivel JC., Lurain N., Reichelderfer P. and Margolis L. Upregulation of Human cytomegalovirus by HIV- in human lymphoid tissue ex vivo. (submitted for publication to AIDS research and human retroviruses)
. &#;oki&#; V.P., Bhattacharya B., Smith R.D., Biancotto A., Noguchi C.T., Puri R.K., and Schechter A.N.. Gene expression profiling in fetal and adult definitive erythropoiesis. (submitted for publication to BMC genomics)
. Biancotto A., Grivel JC., Lisco A., Vanpouille C., Markham P., Gallo R., Margolis LB., and Lusso P. Evolution of SIV toward RANTES Resistance in HHV-A-Coinfected Macaques Rapidly Progressing to AIDS (submitted for publication Nature medicine)
. Biancotto A., Lisco A., Vanpouille C., Margolis LB., and Grivel JC. Activation and Memory phenotype in lymphoid tissue: a model for immune response. (in preparation)
INVITED PARTICIPATIONS AND LECTURES
Invited speaker, First annual Think Tank HIV meeting, NIH,
Presentation title: “Immune dysregulation upon HIV infection in human lymphoid tissue”
Bethesda. Maryland, September ,
Invited participant, CFAR meeting, Center for AIDS Research, Case Western Reserve University,
Cleveland. Ohio, June -,
Invited participant, CLIC meeting, Center for AIDS Research, Case Western Reserve University,
Cleveland. Ohio, July -,
Invited speaker, ISHEID meeting.
Presentation title: “HHV- induced evolution of SIV: Guilt by association”
Toulon, FRANCE, June -,
Invited participant, Center For Aids Research Meeting, Case Western Reserve University,
Cleveland. Ohio, June -,
Invited speaker, Ninth Annual HIV Drug Resistance Program Think Tank.
Presentation title: “In vivo HHV- induced evolution of SIV”
Frederick, Maryland, April ,
Invited speaker, Cleveland Immunology Consortium (CLIC) meeting, Center for AIDS Research, Case Western Reserve University. Presentation title: “Abnormal cytokine profile in HIV infected lymph nodes”
Cleveland. Ohio, Nov -,
Invited speaker, International AIDS Society meeting.
Presentation title: “Dual Role of Prostratin in HIV infection”
Paris, FRANCE, July -,
FELLOWSHIPS AND AWARDS
- -: Fellowship from the Ministère de la Recherche et Technologie.
- -present: Fogarty International Center: Visiting Fellow Fellowship.
- -Bill and Melinda Gates Foundation scholarship (keystones symposia)
REFERENCES
Dr Jean Charles Grivel
NASA NIH Center for Dimension Tissue Culture
LCMB/NICHD/NIH
Bldg. , Room D
Center Dr.
Bethesda, MD
Tel.
Fax:
Email:
Dr Ivan Hirsch
Institut de Recherche sur le Cancer de Marseille, UMR INSERM
Institut Paoli-Calmettes
IFR , Institut de Cancérologie et d'Immunologie de Marseille,
Université de la Méditerranée
, boulevard Lei Roure
MARSEILLE (France)
Tel. +---
Fax. +---
Email:
Dr Leonid Margolis
NASA NIH Center for Dimension Tissue Culture
LCMB/NICHD/NIH
Bldg. , Room D
Center Dr.
Bethesda, MD
Tel.
Fax:
Email:
