Research Fellow Resume
RESUME
SANJEEV KUMAR, Ph.D.
National Institute of Allergy and Infectious Diseases (NIAID),National Institutes of Health (NIH), Twinbrook Parkway,Rockville, MD . Phone: , Fax: , Cell: .
E-mail: . Webpage: http://www.niaid.nih.gov/dir/labs/lmvr/lmvr.htm
EDUCATIONS:
. Ph. D. Molecular Biology, , Banaras Hindu University, India (Advisor: Dr. Rabidranath Chakrabarti).
Thesis title: Signalling events leading to T-cell activation: proliferation and its modulation by Amphotericin B.
. MS. Biotechnology, , Guru Nanak Dev University, India (Advisor: Dr. S. S. Bhullar).
Dissertation title: Purification and characterization of lectins from two Amaranthus species.
. BS. Zoology, Botany, Chemistry, , Meerut University, India.
RESEARCH AND PROFESSIONAL EXPERIENCES:
National Institutes of Health (Dec - Present)
Employed as full-time equivalent (FTE) Research Fellow to study the regulation of Plasmodium (malaria parasite) development inside the mosquito vector. Investigating the role of mosquito innate immune pathways, cellular immune responses and the role of immune genes by Confocal microscopy and double stranded RNA (dsRNA)-mediated gene silencing. Collaborating with senior scientists in the field to develop and evaluate the advanced research in disease transmission. Advisor: Dr. Carolina Barillas-Mury.
Colorado State University, Fort Collins, CO (Dec - Nov )
Post Doctoral Fellow, appointed to elucidate the immune mechanism of malaria vector during Plasmodium transmission. Investigated the role of mosquito reactive oxygen species during Plasmodium infection by Confocal microscopy, biochemical assays and gene expression by real time PCR. Advisor: Dr. Carolina Barillas-Mury.
INSERM-CNRS Centre d'Immunologie Marseille-Luminy (CIML), Marseille, France (Feb - Nov, )
Employed as INSERM foreign scientist to study the mechanism of V(D)J recombination during T cell development. Investigated the mechanism by which a promoter enables transcription to switch on/off and the role of an enhancer to regulate promoter activation during somatic recombination by various genomic DNA foot printings, Electro Mobility Shift Assays (EMSAs) and immunoprecipitation. Advisor: Dr. Pierre Ferrier.
Banaras Hindu University, India ()
Pre-doctoral fellow to study T cell receptor (TCR)-mediated signal transduction during T cell activation. Investigated the role of calcium release from internal stores and regulation of calcium influx by phosphoinositide (PIP)-derived secondary messenger (DAG: Diacylglycerol) during T cell activation. I also studied Amphotericin B-mediated immunomodulatory effect on T cell proliferation. Advisor: Dr. Rabindranath Chakrabarti.
Guru Nanak Dev University, India ()
I carried out one year laboratory work for my MS dissertation in Lectin biology. I purified Lectins (sugar binding glycoproteins) from the seeds of two different species of a plant Amaranthus and studied their immunogenicity, cross reactivity and the effect of divalent cations on their agglutination activity. Advisor: Dr. S. S. Kamboj.
HONOURS AND AWARDS:
. NIH Employee performance awards (, and ).
. National Institutes of Health (NIH) research fellowship, -present.
. Post-doctoral fellowship, Colorado State University, -.
. INSERM fellowship for foreign scientists, France, Feb-Nov, .
. Outstanding achievement Post-doc category poster awards, Colorado State University, and .
. Graduate research fellowship, Government of India, .
. Gold Medal for outstanding achievements during MS degree, Guru Nanak Dev University, India, .
. Student fellowship during MS degree, Government of India, .
SKILLS:
.Expert in biological imaging. Excellent knowledge of light-, digital- and Confocal-microscopy. Skilled user of Leica SP and Olympus Confocal microscopes, which include acquiring the image stacks in different channels ( possible channels at a time in Leica) and their further processing. Since year (starting of my post doctoral training in malaria research) major part of my study is focused on Confocal microscopy (Please see digital and Confocal images in following publications: J. Biol. Chem. . : ; PNAS USA. . : -; Cell. . : -).
.Expert in Deconvolution (a program that removes out-of-focus light from a z-series of images obtained on a wide field fluorescence microscope), IMARIS (a high quality software package to process the images) and Photoshop to refine, merging the Confocal stacks, flattening the images, surpass, -D views and making movies based on the obtained Confocal sections.
. Excellent working knowledge of computer, Internet, MS office, EndNote, Sigma-Plot, SigmaStat and other common computer software.
. Very good knowledge of bioinformatics, DNA Star and Sequencher software.
. Experimental experiences in Immunology, Cell Biology, Molecular Biology, Microbiology, and Vector Biology. Such as, in vitro mammalian/insect cell and Plasmodium culture, cell purification and separation, drug testing in cell culture, nucleic acids (DNA, RNA) purification, RT-PCR, real-time PCR, various DNA foot printings, Gene cloning, DNA sequencing, small interfering RNA (siRNA) and gene silencing, microinjections, biochemical and enzymatic assays, in vivo and in vitro protein synthesis, His-tag protein purification, immunization, antibody production, DNA vaccination, chromatography, ELISA, Southern and Western blotting, immunostaining, immunohistochemistry, EMSA, handling of radioactive and non radioactive probes, cryopreservation and maintenance of biological samples, Plasmodium infection and related techniques.
. Excellent writing skills of research articles and reviews. Expert in oral presentations and scientific interactions.
. Excellent abilities to solve scientific issues, designing and executing research experiments, data analysis, guiding and managing with research students and fellow colleagues.
. Advanced English speaking, reading and writing abilities.
ADDITIONAL RESEARCH TRAININGS, DEMONSTRATOR AND TEACHING EXPERIENCES:
. European Molecular Biology Laboratory (EMBL), Heidelberg, Germany (). Research training in Vector Biology to study the role of reactive oxygen species in Plasmodium refractoriness. Advisor: Prof. Fotis C. Kafatos, Director General of EMBL.
. Regional Research Laboratory (RRL), Jammu Tawi, India (). Summer training in Genetic Engineering. I learned several molecular biology techniques while working on the isolation and characterization of the genes involved in ascorbic acid metabolism in Erwinia herbicola bacterium. Advisor: Dr. G. N. Quazi.
. All India Institute of Medical Sciences, New Delhi, India (). Attended a training program in Flowcytometry. I learned to operate FACScan and analyzing the data. Trainer: Prof. S. N. Das.
. Banaras Hindu University, India (). Participated as demonstrator in scientific workshop. I delivered lectures and trained immunological labs procedures to several participants.
. Polytechnic Institute, India (). Served as guest lecturer. Delivered lectures on immunity/vaccination, taught hours Biochemistry course and guide laboratory practical to Pharmacy classes.
SELECTIVE TALKS AND POSTER PRESENTATIONS:
. ‘Anopheles midgut peroxidase : a major determinant of Plasmodium susceptibility’ Talk presented in Malaria and Vector Biology Group Seminars, NIH, Rockville, MD, Oct. .
. ‘Outlines of current findings in malaria vector biology’ Talk presented in Vector Encounter Meeting, John Hopkins University, Baltimore, MD, July .
. ‘Cell biology of Plasmodium falciparum midgut invasion of Anopheline mosquitoes’ Poster presented in The ASTMH Symposium, Washington, D.C. Nov. .
. ‘Relevance of Time Bomb Model in human malaria transmission’ Talk presented in The ASTMH Symposium, Miami, FL, Nov. .
. ‘Induced peroxidase(s) mediate(s) protein nitration in the Plasmodium ookinete-invaded Anopheles midgut epithelial cells’ Poster presented in Keystone Symposium, Taos, NM, Feb. .
. ‘Plasmodium-induced peroxidases mediate protein nitration in Anopheles midgut epithelium’ Poster presented in Poster Competition Session, Colorado State University, Feb. . Outstanding Award Recipient.
. ‘NOS induction is necessary but not sufficient to mediate midgut nitration in response to Plasmodium invasion in Anopheles mosquito’ Talk presented at th Annual Phi Zeta Research Day, Colorado State University, Jan. .
. ‘Cell biology of ookinete invasion in different Plasmodium-vector combinations’ Poster presented in Poster Competition Session, Colorado State University, Feb. . Outstanding Award Recipient.
. ‘Mechanism of protein nitration and cell death in ookinete-invaded midgut epithelial cells’ Talk presented in The ASTMH Symposium, Denver, CO, Nov. .
. ‘Characterization of signal transduction cascade(s) mediating the immune- and antioxidative-responses in malaria vector Anopheles gambiae’ Poster presented in Keystone Symposium, Taos, NM, Feb. .
. ‘Amphotericin B inhibits Concanavalin A-induced murine T-cell proliferation through the production of HO’ Poster presented in th Annual Meeting of Society of Biological Chemists, Jawaharlal Nehru University, India, Dec. .
PUBLICATIONS:
. DeJong, RJ, Miller LA, Molina-Cruz A, Gupta L, Kumar S, Barillas-Mury C. . Reactive oxygen species detoxification by catalase is a major determinant of fecundity in the mosquito Anopheles gambiae. Proceedings of the National Academy of Sciences (PNAS) USA. : –.
. Barillas-Mury C, Kumar S. . Plasmodium-Mosquito interactions: a tale of dangerous liaisons. Cellular Microbiology. : - (Invited review).
. Kumar S, Barillas-Mury C. . Ookinete-induced midgut peroxidases detonate the Time Bomb in anopheline mosquitoes. Insect Biochemistry and Molecular Biology. : - (Invited review).
. Gupta L, Kumar S. Han YS et al. . Midgut epithelial responses of different mosquito-Plasmodium combinations: the actin cone zipper repair mechanism in Aedes aegypti. PNAS USA. : -.
. Danielli A, Barillas-Mury C, Kumar S, et al. . Overexpression and altered nucleocytoplasmic distribution of Anopheles ovalbumin-like SRPN serpin in Plasmodium-infected midgut cells. Cellular Microbiology. : -.
. Kamhawi S, Ramalho-Ortigao M, Pham VM, Kumar S et al. . A role for insect galectins in parasite survival: Leishmania major persists in its vector Phlebotomus papatasi through binding to PpGalec. Cell. : -.
. Kumar S, Gupta L, Han YS et al. . Inducible peroxidases mediate nitration of Anopheles midgut cells undergoing apoptosis in response to Plasmodium invasion. Journal of Biological Chemistry. : -.
. Spicuglia S, Kumar S, Chasson L et al. . Potassium permanganate as a probe to map DNA-protein interactions in vivo. Journal of Biochemical and Biophysical Methods. : -.
. Kumar S, Christophides GK, Cantera R et al. . The role of reactive oxygen species on Plasmodium melanotic encapsulation in Anopheles gambiae. PNAS USA. : -.
. Spicuglia S, Kumar S, Yeh JH et al. . Promoter activation by enhancer-dependent and -independent loading of activator and coactivator complexes. Molecular Cell. : -.
. Yeh JH, Spicuglia S, Kumar S et al. . Control of IL-R alpha gene expression: structural changes within the proximal enhancer/core promoter during T-cell development. Nucleic Acids Research. : -.
. Chakrabarti R, Kundu S, Kumar S et al. . Retinol catalyses the reduction of MTT to formazone by ascorbic acid. Journal of Cellular Biochemistry. : -.
. Chakrabarti R and Kumar S. . Diacylglycerol mediates T-cell receptor-driven Ca+ influx in T-cells by a novel mechanism independent of protein kinase C activation. Journal of Cellular Biochemistry. : -.
. Kumar S, Chakrabarti R. . Amphotericin B both inhibits and enhances T-cell proliferation: inhibitory effect is mediated through HO production via cyclooxygenase pathway by macrophages. Journal of Cellular Biochemistry. : -.
. Kumar S, Chakrabarti R. . Stimulant-free preculture in heterologous serum-supplemented medium induces unresponsiveness of T-cells to subsequent mitogenic stimulation. Journal of Cellular Biochemistry. : -.
. Kumar S, Chakrabarti R. . [-(Diethylamino)octyl-,,-trimethoxybenzoate HCl] the inhibitor of intracellular calcium mobilization blocked mitogen-induced T-cell proliferation by interfering with the sustained phase of protein kinase C activation. Journal of Cellular Biochemistry. : -.
. Chakrabarti R, Kumar S, Chakrabarti R. . Relative roles of T-cell receptor ligands and interleukin- in driving T-cell proliferation. Journal of Cellular Biochemistry. : -.
VISA STATUS: I have valid EAD (Employment Authorization Document) to work with any employer and my permanent residency (Green Card) adjustment status is at final stage of approval. Currently working on NIH sponsored HB.
References: References will be provided on request.
Sanjeev Kumar, Ph.D.
Rockville, MD
Feb ,
